طرائق جديدة للتحليل بالحقن الجرياني المستمر لتقدير بعض الادوية == New continuous flow injection analysis methods for determination of some drugs
Author name:
منتظر محمد جبار
Supervisor name:
الهام نغيمش مزعل
General topic:
Chemistry
Specific topic:
Analytical Chemistry
Degree:
Master
University:
University of Baghdad
Language:
English
University location:
Baghdad
Key words:
- UV-Vis spectrophotometry
- diphenhydramine hydrochloride
- chlorpheniramine maleate
- captopril
- Ayah 6S×1-ST-2D solar cell analyser
Abstract:
This study evaluated captopril (CAP) for arterial hypertension and chlorpheniramine maleate (CPM) and diphenhydramine hydrochloride (DPH) for allergy symptoms. A homemade measuring cell (Ayah 6S×1-ST-2D Solar Cell CFI Analyzer) was utilized to analyze the precipitate from the medications under review. The study compared the new method to UV-Vis spectrophotometric to assess its efficiency, processed the results statistically, and summarized them in order: Part I: Captopril (CAP) was determined by reacting ammonium cerium(IV) sulfate (ACS) with CAP in an acidic medium to form a white, slightly yellowish precipitate, which was measured by reflecting incident light .The Ayah 6S×1-ST-2D solar cell analyzer was used to optimize physical and chemical parameters. The novel approach had a linear range of 0.07-3.0 mmol/L, a correlation coefficient of (0.9983), and a detection limit of 272.5 ng/25 μl. The RSD% was less than 0.2% for n = 8 CAP concentrations of 0.9, 1.5, and 3.0 mmol/L. The approach recovered captopril from three pharmaceutical tablets from various companies with a recovery rate of 97.1039% to 99.8200%. A comparison was done between the new and classical analysis methods. To analyze UV-Vis spectrophotometry at λmax= 207.2 nm, the standard addition method was used with F-test and paired t-test. The two methodologies showed no meaningful difference with 95% confidence in favor of a homemade tool. Part II: Determination of chlorpheniramine maleate (CPM) involved reacting ACS with CPM in an acidic medium to form a white, slightly yellowish precipitate, which was measured by incident light reflecting off the precipitated particles. Some chemical and physical variables were studied and optimized. The linear range was 0.07-5 mmol/L, with a correlation coefficient (r) of 0.9996 and a limit of detection (LOD) of 195 ng/10 μl. The RSD% was less than 0.2% for n = 8 at 1.0, 1.5, and 4.0 mmol/L CPM. The method estimated drug levels in three prescription pills from various businesses with recovery rates of 98.4625% to 99.2400%. A comparison was done between the new approach and the standard addition method for UV-Vis Sp. at λmax = 261 nm using F-test and paired t-test. The two methodologies showed no meaningful difference with 95% confidence in favor of a homemade tool. Part III: DPH Determination The method involved reacting phosphomolypidic acid (PMA) with DPH in aqueous medium to form a white, slightly yellowish precipitate, which was measured by incident light reflecting off the precipitated particles. Some physical and chemical parameters were studied and improved. The linear range was (0.07-9) mmol/L, with a correlation coefficient (r) of 0.9998, and a detection limit (L.O.D.) of 729.55 ng/50µl for the novel approach. The RSD% was less than 0.2% for n = 8 DPH concentrations of 0.1, 4.0, and 10.0 mmol/L. The procedure was successfully applied to three pharmaceutical tablets from various businesses, showing a recovery rate of 97.7437% to 99.7142%. A comparison was made. Compare the new method to UV-Vis Sp. at λmax = 258 nm using the traditional combination method, including F-test and paired t-test. The two methodologies showed no meaningful difference with 95% confidence in favor of a homemade tool.
Full text:
b79a71a0c7.pdf
Summary:
7e22f6c558.pdf
References:
61ad06e9e7.pdf
