تاثير بعض المستخلصات والمخلفات النباتية والحيوانية في اصابة نبات اللوبياء Vigna unguiculata بمرض التعفن الفحمي المتسبب عن الفطر Macrophomina phaseolina (Tassi) Goid. == Effect of some extracts and plant residues and animal manure on charcoal rot disease caused by Macrophomina phaseolina(Tassi) Goid
Author name:
محمود عودة جعفر
Supervisor name:
محمد عامر فياض
General topic:
Agricultural sciences
Specific topic:
Plant - Prevention
Degree:
Master
University:
University Of Basrah - Faculty Of Agriculture - Department Of Plant Protection
Language:
Arabic
University location:
Basrah
First pages:
31T1859 - p.pdf
Abstract:
This study was carried out in Plant Protection Department Labs and Fields of Agriculture College, Basrah University during Nov.2013 - 2014 in order to study the effect of leaves extracts of three plant, Conocarpus, Eucalyptus and Cabbege, as well as, the effect of three types of animal manure (cow, sheep and poultry) for reducing charcoal rot disease caused by Macrophomina phaseolina on cowpea.Six isolates of M. phaseolina were isolated from different host at different regions in Basrah Governorate. Pathogenicity test showed that cowpea isolate was the most virulent against cowpea seedling were disease incidence and disease severity reached to 55.8 and 41.0 % respectively.Laboratory experiments revealed that the Alcohol extract of eucalyptus, conocarpus, and cabbage were effective in inhibition of the growth of Macrophomina phaseolina . The percentage of inhibition reached to 62.4, 60.5 and 52.7 % for eucalyptus ,cabbage and conocarpus extracts respectively .In addition the concentration of (1%) (for all extracts) was more effective in inhibition of the growth of fungus M. phaseolina compared to the concentration of 0.5%.The percentage of inhibition was 79.57% and 38.27% respectively. The results of electrophoresis experiment for the different isolates of M. phaseolina proteins showed a variation among the 6 isolates from different plant in the number and location of protein bands.In pots experiment also it was found that animal manure for( cow, sheep and poultry) decreased the incidence and disease severity caused by M. phaseolina as disease incidence and severity reached 77.5 and 75% in treatment of animal manure for cow, poultry compared to 25% in pathogen treatment and the animal manure for sheep was the best in depreciate the percent of infection and severity for fungus which it was 38.3% and 13.8% respectively compared to 81.2% and 33.5% for pathogen treatment while the residues of cabbage leaves gives 45.0% and 13.7% respectively.Field experiment about the effect of some plants residues and animal manure on M. phaseolina explained that all plants residues and animal manure except poultry manure may leads to increase the percentage of germination compared to pathogen treatment and up of germination ratio registered in animal manure(sheep) treatment which it was 98% and in plants residues in cabbage 72% and the results showed that the treatment of conocarpus is the best in depreciate the infection which it was 10.1%and 5.5% respectively compared to 48.2%and 34.4% for pathogen treatment and eucalyptus treatment,animal manure (sheep) registered 19.6%,10.9%,19.9% and 12.5% respectively . It was also noticed that animal manure and plant residues increase NPK content in cowpea leaves, as the content of the leaves of the major nutrients NPK registered, the highest ratio from Nitrogen registered in the treatment of concarpus leaves followed by poultry manure and sheep manure which was 46.6 , 39.1, 38.9 gm/Kg - 1 respectively compared to 31.9 gm/Kg - 1 for pathogen treatment,the highest ratio registrered to Phosphorus in conocarpus leaves treatment which was 3.1 gm/Kg - 1 then poultry manure and sheep manure 2.3 gm/Kg - 1 and 2.1 gm/Kg - 1 respectively compared to 1.5 gm/Kg - 1 for pathogen treatment while the highest ratio to Potassium registered in sheep manure which it was 27.9 gm/Kg - 1 then eucalyptus leaves treatment 25.1 gm/Kg - 1 .
