دراسة مقارنة للمحتوى البلازميدي للبكتريا المخمرة لسكر اللاكتوز للعائلة المعوية والمعزولة من عينات بيئية وسريرية == Comparative Study of Plasmid Content of Lactose Ferment Enterobacteriaceae Isolated From Environmental and Clinical Samples
Author name:
تقي عبد الكريم حميد
Supervisor name:
اسراء عبد الجبار ابراهيم
General topic:
Biology
Specific topic:
Microbiology
Degree:
Master
University:
University of Baghdad
Language:
Arabic
University location:
Baghdad
First pages:
24T3227 - p.pdf
Abstract:
183 bacterial isolate from clinical and environmental samples are collected during the 5 months from 1/9/2014 to 1/2/2015.Which 98 clinical bacterial isolates were isolated from patients (urine, blood, sputum and swabs) from different hospitals in Baghdad city, and also 85 environmental bacterial isolates were isolated from Tigris river water samples which are collected from Al - Shawaka area , soil samples of eastern Baghdad collected from Jadied Shat area , and poultry feces which are collected from poultry farm in Al - Sadar city.All clinical and environmental bacterial isolates were identified on the basis of morphology and biochemical tests.Further Confirmative tests were used to confirm the identification of bacterial isolates by using Vitek - 2 compact system and specific primer to detect 16S rRNA gene. Results of bacteria identification showed the following species : Escherichia coli, Klebsiella pneumoniae, Klebsiella.oxytoca Enterobacter aerogenes, Citrobacter freundii, Serratia marcescens, Raoultella planticola, Chryseomonas luteola, Burkholderia cepacia, Aeromonas, Hydrophis , Streptococcus feacalisThe antibiotic resistance patterns showed that all bacterial species were multidrug resistant.Most clinical E.coli (45isolates) revealed high levels resistance against most antibiotics : (100%) amikacin and ampicillin, (97%) cefepime and ceftazidime, (95%) cetriaxone,(86%) tobramycin, (82%) amoxicillin, (77%) gentamicin, (73%) aztreonam. Also environmental E. coli (28isolates) showed high levels resistance, (100%) ampicillin, ceftazidime, cefepime and tobramycine, (96%) nitrofurantion, (92%) Ceftriaxone, (89%) amoxicillin, (82%) amikacin, (60%) levofloxacin and impenem. All K.pneumoniae showed high levels resistance against most antibiotics, ampicillin, amoxicillin, cefepime and ceftazidime (100%).While 30% resistance in ciprofloxacinAbstractfor clinical K.pneumoniae isolates and 8% resistance was shown in each meropenem and levofloxacin.Results of molecular identification of E.coli isolates by using the specific primer for 16S rRNA gene showed that all isolates of Lactose fermenter contained this gene (100%). Also the same 16S rRNA gene was found in the bacteria Citrobacter freundii, Enterobacter aerogenes, Serratia marcescens, Raoultella planticola with the same molecular weight (544 base pair) that found in E. coli isolates. While K.pneumoniae isolates showed two bands for the same 16S rRNA gene with molecular weight of 544 and > 1500 bp.The plasmid DNA analysis by using alkaline lysis method showed that 35/48 (75%) clinical and environmental bacterial isolates contained multiple plasmids their size 1oooo bp, and 15/48 (31.25 %) isolates contained plasmids with molecular weight more than 10000 bp.The leaf extracts of medical plants, Eucalyptus incrassate, Olea eurepea, and Trigonella foenum - graecum L. used as agents to cure the plasmid via adding the plant extract to the culture media or with mixing it with the plasmid DNA. Results showed that both two methods give clear effect
