دراسة تاثير ليزر النديميوم ياك (Nd+3 : YAG) النبضي على خلايا دم الانسان خارج الجسم == Study ofQ - Switched Nd+3 : YAG Laser Effect on Human Blood Cell in Vitro
Author name:
منى جبر لفتة
Supervisor name:
حسن حمادي محمد | مزاحم محمد عبد الله
General topic:
Physics
Specific topic:
Life Physics
Degree:
Master
University:
University Of Basrah - College Of Science
Language:
Arabic
University location:
Basrah
First pages:
26T1693 - p.pdf
Abstract:
Nd+3 : YAG laser wavelength(1064)nm and the two other wavelength, namely λ=532nm and λ =355nm,were used. Many blood sample groups were exposed to these laser wavelengths to study the physical and biological effects appeared as a result of interaction of laser beams with human blood cells. Variable energy densities of these laser wavelength extended from (53.11mJ/cm2 to1990.45mJ/cm2) were employed in these experiments. Sixty human blood samples were collected which included mails with age between(20 - 35)years and weights between (60 - 75)gm. These samples were distributed into three groups : (a) The first group : Twenty human blood samples were exposed to laser (λ=355nm) with energy densities extended from (53.11mJ/cm2 to 1328.18mJ/cm2). The exposure time was fixed to (100sec) and the laser repetition frequency was (5Hz).White blood cells were counted before (control sample) and after laser exposure. types of WBC revealed, fluctuation in the count rates which didn’t reach the level of significant effect. The red blood cells results revealed a numerical changes in the Packed cell volume (PCV) depending on laser power densities. These Changes indicate that the laser affected the cells visbility . On the other hand, The spectroscopic results have been influenced, the optical properties of hemoglobin blood. The decrease in absorbance of hemoglobin and oxyhemoglobin as a function of laser influence gives a further evidence of changes in optical properties as compared with the control samples.(b)The second group : These group samples were exposed to laser wavelength (λ=532nm) with laser, energy densities range extended from (79.62mJ/cm2
