دراسة نسيجية لجلد اناث وذكور الفئران بعد اعطاء عقار الكلوكوزامين سلفيت == Histological Study On The Male And Female Mice's Skin After The Administration Of Glucosamine Sulphate
Author name:
معن ماجد صالح السامرائي
Supervisor name:
عبد الامير حمادي النعيمي
General topic:
Medicine
Specific topic:
Anatomy and Histology and Embryos
Degree:
Master
University:
Mustansiriyah University
Language:
English
University location:
Baghdad
Key words:
- Skin
- glucosamine sulphate
- glycosaminoglycans
First pages:
19T1159 - p.pdf
Abstract:
يتكون الجلد وكما هو معروف جيدا من عدة طبقات من الخلايا متجددة باستمرار عبر اعادة بناء نفسها ذاتيا مرة كل ثلاثون يوما من خلال دورة حيلتية متكررة وتقسم هذه الطبقات الى نوعين اساسية البشرة والادمة. يعتبر الكلوكوز امين سكر اميني بسيط ينبعث من عمليات الايض ال | It is well known that the skin is made up of multiple layers of cells that fare constantly going through self shedding and regeneration once every 30 days, or so in repeated cycles. The layers can be broadly divided into two sections : the epidermis and the underlying dermis. Glucosamine is an amino monosaccharide derived from cellular glucose metabolism. Glucosamine is a simple component or "building block" of more complex molecules. Glucosamine was considered to be an effective treatment for many joint diseases especially osteoarthritis. It is believed that glucosamine maintain healthy joint functions and rebuild damaged joint cartilage, tendons, ligaments and other connective tissue. It does this by stimulating the production of glycosaminoglycans (GAG’s) which are the structural components of cartilage and connective tissue else where in the body. On that basis, this study was designed to study the effects of glucosamine sulphate on the skin.Twenty male and twenty females adult mice (weighting between 23.3gm to 27.2gm), were divided into two equal subgroups (control and experimental); the drug was given at noon three hours after starvation it had been grinded and mixed with food and given as a single oral dose of 350mg/kg body weight per day for 35 successive days. Histological examination and statistical analysis of multiple sections of ventral and dorsal skin of male and female mice and of both subgroups were done by using hemotoxyline and eosin stain. Special stains were used to demonstrate the specific components of the skin as follows : for collagen fibers (Van Gieson's staining method), for elastic fibers (modified Taenzer - Unna Orcien staining method), and for ground substance (Alcian blue stain method).The results reveal that there was no particular change in the epidermis between the experimental and control groups. On the contrary there was a marked thickening with high cellular contents of both layers of the dermis (papillary and reticular).The dermis of the treated animals contains more collagen and elastic fibers. The predominant cell of the dermis was the fibroblast which was more obviously seen in the ventral skin of the treated animals. This study confirms practically that glucosamine sulphate induces significant structural changes in the skin of male and female mice. Using glucosamine sulphate clinically for medical conditions rather than arthritic diseases is now the target of most recent researches, its ability to decrease wrinkles in the aged skin and promotion of wound healing with less scar tissue is consider to be a light for dermatologist and plastic doctors, plus its effect to rebuild any aged, injured and diseased tissue in the body.
