التغيرات البايوكيميائية والهرمونية والتكامل العظمي لزرعات الاسنان في ذكور الارانب الناضجة المستحدث فيها نقص او فرط هرمون الغدة جار الدرقية == Biochemical, Hormonal Alterations and Osseointegration of Dental Implants in Hypo/ Hyperparathyroidism Induced Mature Male Rabbits
Author name:
حسين شاطي العيس
Supervisor name:
نورس عبدالاله علوان | ايمان المسعودي
General topic:
Veterinary Medicine
Specific topic:
Physiology
Degree:
Doctorate
University:
University Of Basrah - College Of Veterinary Medicine - Department Of Physiology, Pharmacology And Chemistry
Language:
English
University location:
Basrah
Key words:
- Calcium
- Parathyroid hormone (PTH)
- hypoparathyroidism
- Proton Pump Inhibitors (PPI)
- Hypomagnesemia
- Osseointegration
First pages:
T96068 - p.pdf
Abstract:
In rabbits, the absorption of calcium is directly correlated with the quantity consumed in the meal, rather than being regulated based on metabolic requirements, and calcium absorption is relatively independent of vitamin D3. In current study try to throw light on biochemical, hormonal, and osseointegration of dental implants in hypo/hyperparathyroidism induced in mature male rabbits. Two experiments have been carried out, In the first experiment; fifty mature male rabbits were randomly divided into 5 groups, (10 rabbits/group); Group 1:(Control); was intraperitoneal (I/P) injected normal saline (1ml/kg/day for 45 days). Group 2: (Hyperparathyroidism); was IP injected with Lithium Chloride (72mg/kg/day for 45 days). Group 3: (Hypoparathyroidism); was IP injected with Omeprazole (PPI) (1mg//kg/day for 45 days). Group 4: (Calcium administered group); was IP injected with Ca2+ (96mg/kg/day for 45 days) Group 5: (Calcium + Vit.D3 administered group); was IP injected with Ca2+ (96mg/kg/day for 45 days) I/P injection and vitamin D3 (10000IU/kg/day for 45 days) orally. In the 2nd experiment, two rabbits from each group received one dental implant in its left tibia (tibial implants) to assist the osseointegration in the studied groups after three months by Computed Tomography scan (CT scan) (Housnfield Unit) and histopathology. At the end of 1st experiment, net body weight (BW) and BW gain was measured and blood samples were collected by cardiac puncture for the biochemical and hormonal assessment. Serum PTH concentration increased significantly in hyperparathyroidism group. While, significantly decreased in hypoparathyroidism, and Ca2++Vit-D3 groups; as compared to control and Ca2+group. All serum mineral levels showed significant decrease in hypoparathyroidism group. While, serum concentration of calcium increased significantly in the hyperparathyroidism group as compared to control and other experimental groups. In the 2nd experiment the results revealed that bone mineral density (BMD) showed significant decrease in hyperparathyroidism group more than control and other experimental groups, and increased significantly in Ca2++Vit-D3, and hypoparathyroidism groups as compared to control, and hyperparathyroidism groups. While, bone mineral content (BMC) showed significant decrease in hyperparathyroidism group as compared to control, and other experimental groups, furthermore significant increase was shown in hypoparathyroidism group as compared to hyperparathyroidism groups. The microscopic examination of the bone sections in hyperparathyroidism group sections showed dense connective tissue deposition and proliferated fibroblast cells. While in Hypoparathyroidism group showed intimate bone formation in the site of bone – implant interaction, in which there were marked formation of the haversian systems with little fibrous tissue remnants; which considered the best group regarding Osseointegration of tibial implantology when compared with hyperparathyroidism group. According to the results of current study we can conclude that; in spite of that Ca2+ have a unique mechanism for absorption in rabbits, but remain the PTH essential to control Ca2+ homeostasis (calciostat), and a potential risk to developed functional hypoparathyroidism by prolong used of PPI which induced hypomagnesemia, in addition to that Hounsfield units (HU) can be used to provide an assessment of bone density and then evaluate the Osseointegration concept.
Full text:
71ad0fd390.pdf
Summary:
2e6c08a6f2.pdf
References:
e764061d61.pdf
