طرائق طيفية جديدة لتقدير ميتاكلوبرامايد هيدروكلورايد والدوكسي سايكلين هيكلية في المستحضرات الصيدلانية == New Spectrophotometric Methods For The Determination of Metoclopramide Hydrochloride And Doxycycline Hyclate In Pharmaceutical Preparation
Author name:
مروة هاشم عباس
Supervisor name:
عباس نور الشريفي
General topic:
Chemistry
Specific topic:
Chemistry
Degree:
Master
University:
University of Babylon - College Of Science
Language:
Arabic
University location:
Babylon
Abstract:
Include a general introduction, categorization and side effect of drug and | how it affect on the body of human-being. Also the significance of | spectrophotometric methods which include using ultra violet and fluorescence | spectra, in addition to it includes a common introduction and significance of | diazotization and coupling reactions and its application in the determination | of drug in the pharmaceutical preparation as well as the chapter includes a | general introduction, employment and classification of azo dyes, also it involves a common introduction, pharmaceutical importance, side effect, physiscal properties and chemical properties of Metoclopramide hydrochloride (MCP-HCl) and Doxycycline hyclate (DOX-HYC) drugs. | Finally, this chapter contain a brief review for the analytical methods which is used for determination of both drugs. after all the chapter have the aim of research. | Chapter two | Include development a simple and sensitive spectral method to determination of Metoclopramide hydrochloride (MCP-HCl) drug, the method depends on the coupling of diazotized drug with the reagent (DOXHYC) in basic media of sodium hydroxide (NaOH) to form a stable, water soluble and yellow azo dye which exhibit a maximum absorption at wavelength (452)nm, the suggested method obey Beer's law in concetration range (0.1-10) μg.ml-1 with correlation coefficient (0.9997), Sandell's sensitivity (0.009) μg.cm2, molar absorption coefficient (3.84*104) L.mol- 1.cm-1, limit of detection (0.0008) μg.ml-1 and limit of quantitation (0.0027) μg.ml-1. The recovery percentage and relative standard deviation is (99.636) (0.379) respectively. The suggested method was applied on some | pharmaceutical preparations which contain (MCP-HCl) drug, this method was compared with standard method which existing in the british pharmacopoeia and found that the experimental value of (t,f) is smaller than of it's table value, whereas there is no difference between the two methods at confidence level 95%. | Include also determination of Doxycycline hyclate (DOX-HYC) drug in the same spectrophotometric method but in reverse way by using Metoclopramide hydrochloride (MCP-HCl) drug as a reagent at maximum absorption wavelength (452)nm and found that the method obey Beer's law in concetration range (0.6-15) μg.ml-1 with correlation coefficient (0.9995), sandell's sensitivity (0.009) μg.cm2, molar absorption coefficient (5.36*105) L.mol-1.cm-1, limit of detection (0.0056) μg.ml-1 and limit of quantitation | (0.0187) μg.ml-1. The recovery percentage and relative standard deviation is (99.554) (0.711) respectively. This method was applied on some pharmaceutical preparations which contain (DOX-HYC) drug, this method was compared with standard method which existing in the US pharmacopoeia and found that the experimental value of (t,f) is smaller than of it's table value, whereas there is no difference between the two methods at confidence level 95%. | Chapter three | Involve determination of Metoclopramide hydrochloride (MCP-HCl) drug in the spectrofluorometric by using (DOX-HYC) as a reagent depending on diazotization and coupling reaction between diazotized Metoclopramide hydrochloride (MCP-HCl) drug and (DOX-HYC) reagent in basic media of sodium hydroxide (NaOH) to form a stable, water soluble and yellow azo dye ich reveal a maximum fluorescence intensity at emission intensity wavelength (524) and excitation intensity wavelength is (426) and found that the suggested method is obey Beer's law in concetration range (0.001-0.1) μg.ml-1 with correlation coefficient (0.9998), limit of detection (21*10-5) μg.ml-1 and limit of quantitation (65*10-5) μg.ml-1. The recovery percentage and relative standard deviation is (99.954) (0.826) respectively. The suggested method was applied successfully on some pharmaceutical preparations which contain (MCP-HCl) drug. | Involve also determination of Doxycycline hyclate (DOX-HYC) drug in the same spectrofluorometric method but in reverse way by using Metoclopramide hydrochloride (MCP-HCl) drug as a reagent at emission intensity wavelength (524)nm and and excitation intensity wavelength is (426) found that the method obey Beer's law in concetration range (0.004-0.6) μg.ml-1 with correlation coefficient (0.9996), limit of detection (72*10-5) μg.ml-1 and limit of quantitation (21*10-4) μg.ml-1. The recovery percentage and relative standard deviation is (100.151) (0.599) respectively. The suggested method was applied successfully on some pharmaceutical | preparations which contain (DOX-HYC) drug.
