تاثير مستخلص بذورالحبة السوداء في امراضية طفيلي المتورقة العملاقة Fasciola gigantica خارج وداخل الجسم الحي == Efficiency of Nigella Sativa Seed Extract In Fasciola Gigantica Parasite In Vivo And In Vitro
Author name:
شيماء عبد الحسين محمد شلاش
Supervisor name:
جاسم حميد رحمة الخزاعي
General topic:
Biology
Specific topic:
Microbiology
Degree:
Doctorate
University:
University of Kufa - College Of Science - Department Of Biology
Language:
English
University location:
Najaf
First pages:
24T2761 - p.pdf
Abstract:
اجريت الدراسة الحالية خلال المدة من شهر تشرين الثاني 2012 ولغاية تشرين الاول 2013لتقييم فعالية المستخلص الكحولي لبذورالحبة السوداء Nigella sativaفي حيوية بيض وبالغات دودة الكبد العملاقة Fasciola gigantica خارج وداخل الجسم الحي. وبواقع ثلاث تراكيـز 20%, 4 | The present study was conducted during the period from November 2012 till October 2013 to evaluate the effectiveness of the alcoholic extract for Nigella sativa seeds on the vitality of eggs and adults liver giant worm Fasciola gigantica In vitro and In vivo in the infected domestic rabbits lepus lepus arabica. Alcoholic extract for Nigella sativa seeds used In vitro at three concentrations 20%, 40%, 60% for each eggs and adults of Fasciola gigantica and In vivo were three doses 200, 400, 600 mg/kg from body weight in the infected rabbits by this parasite. The result of the current study In vitro revealed that the alcoholic extract for Nigella sativa seeds at 60% has been great effects in reducing the percentage of the eggs hatching to 0% and increasing the mortality percentage of the adult worms to 100% when compared with the control groups. So the results of this study showed that there is not significant differences of alcoholic extract of Nigella sativa seeds on the blood components of the healthy rabbits at level P<0.05 while in the infected and treated rabbits there are less significant effect when compared with the infected rabbits. In the infected rabbits showed significant increased in numbers of white blood cells from 5.52*109/L in the negative control to 10.41*109/L in the positive control and significant decreased in numbers of red blood cell from 5.51*1012/L to 4.41*1012/L and haematocrit value from 32.21% to (24.71%) and the amount of hemoglobin from 11.43*gm/dl to 8.53* gm/dl.While the ethanol extract of Nigella sativa seeds reduced the number of WBCs and increased the number of RBCs, PCV and the concentration of hemoglobin. Also the results of the current study In vivo showed that the ability of alcoholic extract of Nigella sativa seeds at dose 600 mg/kg are more effective in reducing the numbers of Fasciola gigantica worms in the infected and treated rabbits when compared with the infected and untreated rabbits. So the total rate number of worms in the positive control 16.7 while 6.4, 2, 0 for doses 200, 400, 600 mg/kg from body weight respectively. Results of histological study in the experimental infected rabbits revealed that the alcoholic extract is very effective for reducing the histopathological changes in the liver, spleen and kidney which caused by the parasite. And the therapeutic efficiency of these extract in dose 600 mg/kg for organs Liver, Spleen, Kidney are 100%. Concluded from the results of this study that the alcoholic extract of Nigella sativa seeds used in treatment the infection of the F.gigantica worm because its therapeutic efficiency and not any side effects to this extract.
