العلاقة بين تعدد النمط الوراثي للجين GSTP1 ومرض ابيضاض الدم النخاعي الحاد لمرضى عراقيين == Association of Glutathione - S - Transferas (GSTP1) gene polymorphism with Acute Myeloid leukemia in Iraqi patients
Author name:
ميادة خالد ابراهيم
Supervisor name:
بتول علي شهاب
General topic:
Biology
Specific topic:
zoology - Genetics
Degree:
Master
University:
University of Baghdad
Language:
English
University location:
Baghdad
First pages:
24T3431 - p.pdf
Abstract:
This study was carried out to investigate the relationships between acute myeloid leukemia (AML) patients and the genotyping of Glutathione S - Transferase P1 class (GSTP1) by using Polymerase Chain Reaction (PCR) - Restriction Fragment Length Polymorphism (REFLP) and measurement of some clinical parameters of patients and control.This study included 120 blood samples for Iraqi individuals. Sixty individuals with AML were diagnosed by the consultant of The National Center of Hematology medical City, Center for Hematology AL - Mustanseria University, hematology clinic of Baghdad Hospital and Alkadhymian medical city teaching hospital during the period from October 2014 to May 2015. Those AML cases then have been diagnosed by a specialized hematologists depending on bone marrow aspiration, biopsies reports and other diagnostic criteria for AML according to the International Staging System (ISS).Sixty individuals healthy also included as control group, their age range were between 15 - 70 years. Blood were used as samples to found the correlation between genotyping of GSTP1 and all studied parameters.Molecular studies involved DNA extraction and PCR - RFLP was carried to detect the genetic polymorphism of GSTP1 for all individuals of this study. Molecular study was conducted in the laboratory of the Biology Department / College of Sciences for women. The results showed that in the age for patients and control group were no significant difference in group less than 30 years old, while showed significant difference (P˂0.05) between other groups 30 - 50 years and 50 - 70 years, were (30%) (38.30%) (33.3%) and (23.0%) respectively.The results showed that there were significant different between patients (10.40±2.97) and controls (6.25±0.37) in the mean of white blood cells count (WBCc) (P˂0.48), the mean of hemoglobin (Hb) in patients was (8.11±0.37) and in control was (12.81±1.31) and the different was highly significant between them.The results of genetics polymorphism of the GSTP1 showed the significant difference (P˂0.05) between patients were (56.67%) and controls were (65.00%) in wild genotype AA, while the heterozygous genotype AG and homozygous mutant genotype GG were in AML patients and in controls were no significant different.Distribution of GSTP1 polymorphism and age of patients groups showed that there were significant difference between all age groups and all types of gene polymorphism, were (38.24%), (32.35%), (29.41%) in AA genotype, in AG were (26.32%), (21.05%), (52.63%) and in GG were (57.14%), (42.86%) and (0.00%) respectively.The results of relationship between the sex and AML patients showed that mutant genotype GG genotype was more in male (85.71%) than female (14.29%) with highly significant increase and the risk of male to be effect was more 1,5 than female. The mutant allele G is more frequent in male (0.38) than female (0.17). The data also showed no significant difference between the GSTP1 polymorphism and the mean value of WBC, but the mean value of Hb was shown significant different (P˂0.05), the heterozygous genotype AG showed the highest value (11.35± 0.73).Distribution of GSTP1 polymorphism between control groups according to non - smoking and smoking in the genotype AA+AG, (93.33%), (90.00%) respectively, showed significant difference (P<0.05), while in genotype AG+GG (26.67%), (43.33%) showed high significant difference (P<0.01) and the mutant allele G was more frequency in smoker control (0.27) but in non - smoker was (0.17).Results of this study suggest that GSTP1 gene polymorphisms was not associated with AML disease and GSTP1 has no active role in the pathogenesis of AML, while the age and sex may have a risk factor for progression AML
