تاثير التلقيح ببعض الفطريات المعزولة من الترب الزراعية في المعالجة الحيوية للترب الملوثة بالمخلفات النفطية في محافظة البصرة == Effect of inoculation by some fungi isolated from agricultural soils in the bioremediation of contaminated soils with oil waste in Basrah
Author name:
هدى حاكم سعدون الاسدي
Supervisor name:
ميعاد مهدي الجابري
General topic:
Agricultural sciences
Specific topic:
Soil and Water
Degree:
Master
University:
University Of Basrah - Faculty Of Agriculture
Language:
Arabic
University location:
Basrah
First pages:
31T1880 - p.pdf
Abstract:
For the purpose of identifying the efficiency of inoculation different genus of fungi isolated from agricultural areas in the bioremediation of soil contaminated with petroleum hydrocarbon nearby regions from some the oil fields in the province of Basrah, southern Iraq, and the impact on plant growth as has been selected sites west of Qurna fields and North Rumaila fields, mixed with non - contaminated soil from the same areas at rates of 100% and 75% and 50% to reduce the pollution of oil hydrocarbons ratio.Isolation and purification of two species from fungus Aspergillus (A. niger and A. versicolor), Trichoderma viride and Penicillium citrinum from Five of agricultural areas (Shatt Al - Arab, Garma Ali, Al - Zubair, Al - deer and Abu Al - khaseeb) and studied the possibility of its adaptation and its growth in nutritional media contaminated with the quality of the two regions of oil each separately and inoculated the soil treatments that represent percentages above and inoculated at a temperature of 25 ᵒC to study the total numbers of bacteria and fungi and CO2 amount from Activity microbial in soil and estimated the amount of hydrocarbons broken by the inoculation process fungal soil every 10 days for 60 days of incubation.Agricultural experiment carried out by tested the most efficient of the two fungi in degraded hydrocarbons for each soil and for each contamination level in addition the treatment of a mixture of the most efficient of the two fungi and control treatment (without pollination), left after the treatments for two months with maintaining the humidity at around 75% of field capacity in a way to compensate the weight loss and planted them maize seeds Zea mays L. class Bhooth 106 and after 45 days of germination was estimated proportion of hydrocarbons broken and some parameter of plant growth as a rate of plant length, dry weight of the shoot and the quantity N, P and K absorbed in plant. Were obtained the following results : 1. increase the number of the total fungi and bacteria with time in soils inoculated with isolated fungi to Limit 60 days compared to the control treatment in which the increase lasted only 40 days in the pollution levels of 75% and 100%, while the increase continued to 60 days at the level of 50% of all pollination treatments, including the control treatment.2. increase the amount of CO2 liberated from Bioactivity with time in soil inoculated fungal treatment compared with control treatment and fungal isolates varied in their efficiency to stimulate biological activity, and mitigate pollution of soils to the extent of 50% contributed to the increase in the vital activity of soils represented an increase of the amount of the liberated CO2 .3. inoculation contaminated soils fungal isolates led to increase petroleum hydrocarbon ratio analysis with time and varied fungal isolates in their ability to biodegraded hydrocarbon wholly followed the following sequence : Aspergillus niger > Penicillium citrinum > Trichoderma - viride > Aspergillus versicolor in the soil of North Rumaila and sequence : Penicillium citrinum < Trichoderma viride < Aspergillus niger <Aspergillus versicolor in soils west of Qurna fields and gave the pollution level 50% higher analysis of petroleum hydrocarbon ratio compared to levels of 75% and 100%.4. Inoculate all levels of contamination by fungal isolates most efficient led to increasing lengths and dry weight of the plant maize and increase the quantity absorbed N, P and K by the plant and were observed increase in the proportion of hydrocarbons degraded at the end of the agricultural experiment.5. Soil North Rumaila showed a response to the inoculation of fungi in all levels of contamination of soil over Western Qurna fields in terms of the total numbers of fungi and bacteria and amount of the liberated CO2 and hydrocarbon ratio degradation as well as an increase in parameters of all the maize plant growth.6. The fungus Penicillium citrinum and Trichoderma viride Showed more efficient in increasing the activity and bioremediation in soil Western Qurna fields, while the fungus Aspergillus niger is the most efficient in the soil of the North Rumaila
